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Abstract HIV infection is the greatest health crisis in human history. Five new methods are discussed. Intravaginal lime or lemon juice has been used for centuries as a traditional contraceptive. The juice can also kill HIV in the laboratory, but clinical trials are needed to see if vaginal application is acceptable, safe and effective. Monkeys can be protected from Simian immunodeficiency virus SIV infection by keratinizing the vagina with topical oestrogen.

If women take the oral contraceptive pill vaginally it retains its contraceptive efficacy, and the oestrogen it contains should thicken the vagina and protect against HIV infection. Clinical trials are needed. Removal of the inner foreskin removes the main site of HIV entry into the penis, resulting in a sevenfold reduction in susceptibility to infection.

The practice needs to be promoted. Wiping the penis immediately after intercourse with lime or lemon juice or vinegar should kill the virus before it has had a chance to infect.

A clinical trial of efficacy is needed. Currently, there are 35—42 million adults and children living with the infection, with around 5 million new infections and 3 million deaths a year UNAIDS Sub-Saharan Africa is the epicentre of the pandemic, with 25 million currently infected and 3 million new infections annually.

Next comes South and South-East Asia, with 6. There is no doubt that the pandemic is still in its exponential growth phase, and that the situation will inevitably get much worse. But in spite of these grim statistics, there is reason for hope; relatively simple interventions could bring the infection under control, even though we may never be able to eradicate the disease completely.

The virus is a fragile one, with low infectivity. For the infection to spread, R0 must be greater than one; each infected individual must infect more than one other person on average.

The probability of transmission per act of heterosexual intercourse between otherwise healthy individuals may be as low as 1 in Royce et al. Behavioural changes, such as reducing the number of sexual partners, and reducing the age difference between partners, could greatly reduce the rate of transmission. But there is one major obstacle; of all aspects of human behaviour, it is our sexual behaviour that is least amenable to change.

By parasitizing our sexuality, the virus has ensured its propagation. The most widely advocated programme of prevention which is acceptable, at least in part, to most of the world's political and religious leaders is the ABC approach—Abstinence, Be Faithful, and Condoms. But each component has its inherent weaknesses: In practice, promoting abstinence does not work.

Alas, we are not by nature a monogamous species. If we were, then STD would not exist. We can never rely on male compliance to control a disease that is increasingly female-orientated. The advent of antiretroviral drugs can now offer significant improvements in the quality of life and increase in life expectancy to those who are infected.

Even if they only doubled life expectancy, they would be a priceless gift. But the drugs are costly, the daily treatment regimen may be difficult to comply with, there can be serious side effects, and ultimately the high mutation rate of the virus ensures that resistance is inevitable.

These drugs are emphatically not a cure; you will probably still die of an AIDS-related disease. However, as treatments improve and become more widely available, more people are likely to volunteer for HIV testing, and this alone will greatly help HIV prevention.

The problem with antiretroviral drugs is that the virus can mutate far faster than the pharmaceutical companies can develop new therapies, so the virus will always be ahead of the game. This high viral mutation rate also bedevils attempts to produce an anti-HIV vaccine, which is not yet on the horizon, and may never be. After all, we still do not have a vaccine against malaria, after many more years of research.

And we have recently uncovered a nasty twist to HIV. If somebody becomes infected with two different strains of the virus, this greatly accelerates disease progression, and the two strains may even recombine to form a new, resistant strain Gottlieb et al.

So almost a quarter of a century after the first recognition of AIDS, the infection continues to run rampant throughout the globe. We have spent billions of dollars on the development of treatments and the search for vaccines, and we understand the genetic makeup of the virus in minute detail. But we have ignored the development of simple new preventative measures. Surely we should not still be relying on sixteenth century technology as our sole defence against HIV infection?

We need a new ethic to stem the global spread of HIV infection. We must give the highest priority to the development of simple preventative methods that are designed first and foremost for women, and especially for the poor living in the developing world. In order to do this, we need to go back to first principles and ask ourselves four very simple questions. How does the virus enter the female reproductive tract, and how might a woman's natural resistance be increased?

How does the virus enter the male reproductive tract, and how might a man's natural resistance be increased? Let us examine five possible new approaches to HIV prevention that address these questions. Lemon or lime juice: The principal route of entry of HIV into the female reproductive tract appears to be via the Langerhans cells and other dendritic cells in the vaginal epithelium.

It therefore makes sense to try and kill the virus before it can become attached to the specific HIV receptors in the vaginal epithelium. There is general agreement that we urgently need to develop new ways of protecting women from HIV infection that do not depend on the compliance of their male partners Pilcher , and the American-based Microbicides Alliance, with over million dollars at its disposal, is going down the conventional route of drug discovery, patent protection and pharmaceutical company development to test more than 40 new compounds as potential vaginal microbicides Coplan et al.

But the Microbicides Alliance is faced with a major hurdle to overcome before any of their compounds can be licensed for use. The American Food and Drug Administration FDA has insisted that efficacy needs to be tested in a 3-arm clinical trial, comparing the active product with a placebo and with condoms only. None of the new compounds under development can meet these stringent requirements before at the earliest. In the meantime, it is possible that we could take a short-cut by studying traditional compounds that women have been using intra-vaginally in an attempt to regulate their fertility.

We have opted for this approach, studying a natural contraceptive that women have been using for centuries—lemon juice. Norman Himes has given a comprehensive review of the history of contraceptive development throughout the world. He describes how Casanova advocated the use of half a lemon, partially squeezed out, as a cervical cap to protect women from pregnancy—the forerunner of today's cervical caps.

It has long been known that dilute lemon juice is a very effective spermicide, because of the low pH produced by its citric acid content Himes Since the human immunodeficiency virus is also extremely susceptible to low pH Martin et al.

We have been able to confirm the spermicidal effect of both lemon and lime juice Clarke et al. Fresh lime juice, with a pH of 2. So it is easy to see why intravaginal lime or lemon juice is such a very effective contraceptive. Since improved access to Family Planning is seen as one of the keys to poverty reduction in the developing world in the recently announced Millennium Development Goals Sachs , this simple contraceptive deserves to be much more widely known.

We have gone on to study the virucidal effects of lemon and lime juice Short et al. Using filtered pools of lemon juice pH 2. The presence of live virus was determined by its ability to infect peripheral human blood mononuclear cells cultured for 14 days. Viral replication was measured by viral reverse transcriptase activity in culture supernatants. In order to maximize the virucidal effect, it will be necessary to lower the pH of the ejaculate to below 4.

Since the mean ejaculate volume of fertile men is 3. Would undiluted citrus juice placed in the vagina before sex be painful to either partner? A simple clinical trial was carried out in Mexico City in Guiterrez et al. By way of comparison, they were asked to rank the pain score when the juice was applied to the tip of the tongue. They were also asked to record sex, age, and number of prior sexual partners, and men were asked to record their circumcision status.

Sixty one men and 64 women took part in the study. The results showed that the median pain score on application of neat lime juice to the penis or vagina was 0, no different from the pain when applied to the tip of the tongue. Male circumcision was without effect, and there was no effect of age, or number of prior sexual partners. A small number of women did experience moderate discomfort, apparently associated with the onset of menstruation, but in no case was it severe. This is a convincing demonstration of the potential acceptability of intravaginal citrus juice to both partners.

How safe would it be to repeatedly administer neat citrus juice to the vagina? We undertook a clinical trial in 12 cynomolgus monkeys Macaca fascicularis , six treated and six controls, each housed in isolation.

The treated animals had a small cotton wool ball soaked in about 0. The six control animals were not killed, but had vaginal biopsies taken on the first day of menstruation. The vaginal epithelium of the treated animals did not differ histologically from the control animals, and the cervical epithelium appeared normal see figure 1.

Both treated and control animals showed occasional foci of sub-epithelial vaginal infection.

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Episode 2 TOO MUCH DICK?



World sex 2 pussy one dick

Abstract HIV infection is the greatest health crisis in human history. Five new methods are discussed. Intravaginal lime or lemon juice has been used for centuries as a traditional contraceptive. The juice can also kill HIV in the laboratory, but clinical trials are needed to see if vaginal application is acceptable, safe and effective. Monkeys can be protected from Simian immunodeficiency virus SIV infection by keratinizing the vagina with topical oestrogen.

If women take the oral contraceptive pill vaginally it retains its contraceptive efficacy, and the oestrogen it contains should thicken the vagina and protect against HIV infection.

Clinical trials are needed. Removal of the inner foreskin removes the main site of HIV entry into the penis, resulting in a sevenfold reduction in susceptibility to infection. The practice needs to be promoted. Wiping the penis immediately after intercourse with lime or lemon juice or vinegar should kill the virus before it has had a chance to infect.

A clinical trial of efficacy is needed. Currently, there are 35—42 million adults and children living with the infection, with around 5 million new infections and 3 million deaths a year UNAIDS Sub-Saharan Africa is the epicentre of the pandemic, with 25 million currently infected and 3 million new infections annually. Next comes South and South-East Asia, with 6. There is no doubt that the pandemic is still in its exponential growth phase, and that the situation will inevitably get much worse.

But in spite of these grim statistics, there is reason for hope; relatively simple interventions could bring the infection under control, even though we may never be able to eradicate the disease completely.

The virus is a fragile one, with low infectivity. For the infection to spread, R0 must be greater than one; each infected individual must infect more than one other person on average. The probability of transmission per act of heterosexual intercourse between otherwise healthy individuals may be as low as 1 in Royce et al. Behavioural changes, such as reducing the number of sexual partners, and reducing the age difference between partners, could greatly reduce the rate of transmission.

But there is one major obstacle; of all aspects of human behaviour, it is our sexual behaviour that is least amenable to change. By parasitizing our sexuality, the virus has ensured its propagation. The most widely advocated programme of prevention which is acceptable, at least in part, to most of the world's political and religious leaders is the ABC approach—Abstinence, Be Faithful, and Condoms. But each component has its inherent weaknesses: In practice, promoting abstinence does not work.

Alas, we are not by nature a monogamous species. If we were, then STD would not exist. We can never rely on male compliance to control a disease that is increasingly female-orientated.

The advent of antiretroviral drugs can now offer significant improvements in the quality of life and increase in life expectancy to those who are infected. Even if they only doubled life expectancy, they would be a priceless gift.

But the drugs are costly, the daily treatment regimen may be difficult to comply with, there can be serious side effects, and ultimately the high mutation rate of the virus ensures that resistance is inevitable. These drugs are emphatically not a cure; you will probably still die of an AIDS-related disease. However, as treatments improve and become more widely available, more people are likely to volunteer for HIV testing, and this alone will greatly help HIV prevention. The problem with antiretroviral drugs is that the virus can mutate far faster than the pharmaceutical companies can develop new therapies, so the virus will always be ahead of the game.

This high viral mutation rate also bedevils attempts to produce an anti-HIV vaccine, which is not yet on the horizon, and may never be. After all, we still do not have a vaccine against malaria, after many more years of research. And we have recently uncovered a nasty twist to HIV. If somebody becomes infected with two different strains of the virus, this greatly accelerates disease progression, and the two strains may even recombine to form a new, resistant strain Gottlieb et al.

So almost a quarter of a century after the first recognition of AIDS, the infection continues to run rampant throughout the globe. We have spent billions of dollars on the development of treatments and the search for vaccines, and we understand the genetic makeup of the virus in minute detail.

But we have ignored the development of simple new preventative measures. Surely we should not still be relying on sixteenth century technology as our sole defence against HIV infection? We need a new ethic to stem the global spread of HIV infection. We must give the highest priority to the development of simple preventative methods that are designed first and foremost for women, and especially for the poor living in the developing world.

In order to do this, we need to go back to first principles and ask ourselves four very simple questions. How does the virus enter the female reproductive tract, and how might a woman's natural resistance be increased?

How does the virus enter the male reproductive tract, and how might a man's natural resistance be increased? Let us examine five possible new approaches to HIV prevention that address these questions. Lemon or lime juice: The principal route of entry of HIV into the female reproductive tract appears to be via the Langerhans cells and other dendritic cells in the vaginal epithelium.

It therefore makes sense to try and kill the virus before it can become attached to the specific HIV receptors in the vaginal epithelium. There is general agreement that we urgently need to develop new ways of protecting women from HIV infection that do not depend on the compliance of their male partners Pilcher , and the American-based Microbicides Alliance, with over million dollars at its disposal, is going down the conventional route of drug discovery, patent protection and pharmaceutical company development to test more than 40 new compounds as potential vaginal microbicides Coplan et al.

But the Microbicides Alliance is faced with a major hurdle to overcome before any of their compounds can be licensed for use. The American Food and Drug Administration FDA has insisted that efficacy needs to be tested in a 3-arm clinical trial, comparing the active product with a placebo and with condoms only. None of the new compounds under development can meet these stringent requirements before at the earliest. In the meantime, it is possible that we could take a short-cut by studying traditional compounds that women have been using intra-vaginally in an attempt to regulate their fertility.

We have opted for this approach, studying a natural contraceptive that women have been using for centuries—lemon juice. Norman Himes has given a comprehensive review of the history of contraceptive development throughout the world.

He describes how Casanova advocated the use of half a lemon, partially squeezed out, as a cervical cap to protect women from pregnancy—the forerunner of today's cervical caps. It has long been known that dilute lemon juice is a very effective spermicide, because of the low pH produced by its citric acid content Himes Since the human immunodeficiency virus is also extremely susceptible to low pH Martin et al.

We have been able to confirm the spermicidal effect of both lemon and lime juice Clarke et al. Fresh lime juice, with a pH of 2. So it is easy to see why intravaginal lime or lemon juice is such a very effective contraceptive. Since improved access to Family Planning is seen as one of the keys to poverty reduction in the developing world in the recently announced Millennium Development Goals Sachs , this simple contraceptive deserves to be much more widely known.

We have gone on to study the virucidal effects of lemon and lime juice Short et al. Using filtered pools of lemon juice pH 2. The presence of live virus was determined by its ability to infect peripheral human blood mononuclear cells cultured for 14 days. Viral replication was measured by viral reverse transcriptase activity in culture supernatants. In order to maximize the virucidal effect, it will be necessary to lower the pH of the ejaculate to below 4.

Since the mean ejaculate volume of fertile men is 3. Would undiluted citrus juice placed in the vagina before sex be painful to either partner? A simple clinical trial was carried out in Mexico City in Guiterrez et al. By way of comparison, they were asked to rank the pain score when the juice was applied to the tip of the tongue. They were also asked to record sex, age, and number of prior sexual partners, and men were asked to record their circumcision status.

Sixty one men and 64 women took part in the study. The results showed that the median pain score on application of neat lime juice to the penis or vagina was 0, no different from the pain when applied to the tip of the tongue.

Male circumcision was without effect, and there was no effect of age, or number of prior sexual partners. A small number of women did experience moderate discomfort, apparently associated with the onset of menstruation, but in no case was it severe. This is a convincing demonstration of the potential acceptability of intravaginal citrus juice to both partners. How safe would it be to repeatedly administer neat citrus juice to the vagina? We undertook a clinical trial in 12 cynomolgus monkeys Macaca fascicularis , six treated and six controls, each housed in isolation.

The treated animals had a small cotton wool ball soaked in about 0. The six control animals were not killed, but had vaginal biopsies taken on the first day of menstruation. The vaginal epithelium of the treated animals did not differ histologically from the control animals, and the cervical epithelium appeared normal see figure 1.

Both treated and control animals showed occasional foci of sub-epithelial vaginal infection.

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